Control of Gene Expression (Edexcel IAL Biology)

Spec point — 3.18

Control of Gene Expression

• Hormones can alter the events inside a cell by influencing gene expression*

• Eukaryotes use transcription factors to control gene expression

  • A transcription factor is a protein that controls the transcription* of genes by binding to a specific region of DNA

  • It is estimated that ~10 % of human genes code for transcription factors

    • There are several types of transcription factors that have varying effects on gene expression

      • E.g. transcription factors that increase the rate at which a gene is expressed are known as activators, while those that decrease gene expression are known as repressors

    • Transcription factors ensure that genes are being expressed in the correct cells, at the correct time and to the right level

    • Transcription factors allow organisms to respond to their environment

• Some hormones achieve their effect by acting upon transcription factors

Effect of hormones inside cells

• Hormones that can cross the cell surface membrane, e.g. steroid hormones and thyroid hormones, are able to enter the nucleus and bind to transcription factors that are present there

  • Steroid hormones are lipid soluble, allowing them to pass between the phospholipids of the cell surface membrane

• An example of this is the hormonal regulation of body temperature

  • At normal body temperature a transcription factor known as the thyroid hormone receptor binds to a section of DNA at the start of a gene

    • This gene codes for a protein that increases the metabolic rate, generating more heat and therefore increasing body temperature

  • As long as the thyroid hormone receptor is bound to the region of DNA at the start of the gene, the gene will not be expressed; it can be said to be switched off

    • In reality it is more likely that expression of the gene will be reduced rather than switched off entirely

  • However, in cold temperatures the body will release the hormone thyroxine which binds to the thyroid hormone receptor

  • Once the hormone and the receptor are bound together the thyroid hormone receptor allows RNA polymerase to bind to the start of the gene; the gene is said to be switched on and its rate of expression will increase

  • The protein which increases the metabolic rate is produced in larger quantities, leading to an increase in body temperature

The hormone thyroxine acts as a transcription factor by binding to the thyroid hormone receptor; this switches on the gene, allowing it to be transcribed by RNA polymerase

Effect of hormones from outside cells

• Hormones that cannot cross the cell membrane, e.g. protein and peptide hormones, bind to receptors in the cell surface membrane

  • Examples of such hormones include

    • Adrenaline
    • Insulin
    • Glucagon
    • ADH

• The binding of these hormones to cell surface membrane receptors initiates a process that activates messenger molecules in the cytoplasm of the cell known as second messengers

  • A common second messenger molecule is cyclic AMP (cAMP), formed from ATP

• The activated second messenger molecules activate enzymes called protein kinases

• Active protein kinase enzymes trigger a chain of reactions, known as a cascade, inside the cell

• The cascade may result in changes to the activity of transcription factors which may then affect gene expression in the cell

Adrenaline acts by binding to a receptor on cell surface membranes; this activates the second messenger cAMP, leading to a cascade of reactions that affects the activity of the cell, e.g. by influencing transcription factors